Zhu M Y, Zhu M S
Institute of Biophysics, Chinese Academy of Sciences, Beijing.
Sheng Li Xue Bao. 1995 Feb;47(1):73-9.
Changes of calcium and magnesium concentration in human platelets during thrombin induced agglutination and the distribution of the respective concentration in the platelet population were measured by fluorescence probe using fura 2, mag-fura 2 and fluo 3. The cytoplasmic free calcium concentration increases with addition 2 thrombin. When the thrombin concentration was increased to 0.5 U/ml, the calcium concentration increase showed two rising peaks. The popular cytoplasmic calcium concentration in platelets shows normal distribution. The cytoplasmic free magnesium concentration also increases with increase of calcium concentration, showing that magnesium also plays a significant effect in the condensation of platelets.
使用fura 2、mag-fura 2和fluo 3荧光探针测量了凝血酶诱导凝集过程中人类血小板中钙和镁浓度的变化以及各自浓度在血小板群体中的分布。随着凝血酶的加入，细胞质游离钙浓度升高。当凝血酶浓度增加到0.5 U/ml时，钙浓度增加呈现两个上升峰。血小板中普遍的细胞质钙浓度呈正态分布。细胞质游离镁浓度也随着钙浓度的增加而增加，表明镁在血小板凝集过程中也起重要作用。
