Angiolillo A L, Sgadari C, Taub D D, Liao F, Farber J M, Maheshwari S, Kleinman H K, Reaman G H, Tosato G
Department of Hematology/Oncology, Children's National Medical Center, Washington, DC 20010, USA.
J Exp Med. 1995 Jul 1;182(1):155-62. doi: 10.1084/jem.182.1.155.
Human interferon-inducible protein 10 (IP-10), a member of the alpha chemokine family, inhibits bone marrow colony formation, has antitumor activity in vivo, is chemoattractant for human monocytes and T cells, and promotes T cell adhesion to endothelial cells. Here we report that IP-10 is a potent inhibitor of angiogenesis in vivo. IP-10 profoundly inhibited basic fibroblast growth factor-induced neovascularization of Matrigel (prepared by H. K. Kleinman) injected subcutaneously into athymic mice. In addition, IP-10, in a dose-dependent fashion, suppressed endothelial cell differentiation into tubular capillary structures in vitro. IP-10 had no effect on endothelial cell growth, attachment, and migration as assayed in vitro. These results document an important biological property of IP-10 and raise the possibility that IP-10 may participate in the regulation of angiogenesis during inflammation and tumorigenesis.
人干扰素诱导蛋白10（IP - 10）是α趋化因子家族的一员，可抑制骨髓集落形成，在体内具有抗肿瘤活性，对人单核细胞和T细胞具有化学趋化作用，并促进T细胞与内皮细胞的黏附。在此我们报告，IP - 10在体内是一种有效的血管生成抑制剂。IP - 10能显著抑制皮下注射到无胸腺小鼠体内的基质胶（由H. K. Kleinman制备）中碱性成纤维细胞生长因子诱导的新血管形成。此外，IP - 10在体外以剂量依赖的方式抑制内皮细胞分化为管状毛细血管结构。体外实验检测显示，IP - 10对内皮细胞的生长、黏附和迁移没有影响。这些结果证明了IP - 10的一项重要生物学特性，并提出IP - 10可能参与炎症和肿瘤发生过程中血管生成调控的可能性。
