一种通过用重组噬菌体溶菌酶快速裂解从李斯特菌细胞中高效回收DNA、RNA和蛋白质的新方法。
A new procedure for efficient recovery of DNA, RNA, and proteins from Listeria cells by rapid lysis with a recombinant bacteriophage endolysin.
作者信息
Loessner M J, Schneider A, Scherer S
机构信息
Forschungszentrum für Milch und Lebensmittel Weihenstephan, Technische Universität München, Freising, Germany.
出版信息
Appl Environ Microbiol. 1995 Mar;61(3):1150-2. doi: 10.1128/aem.61.3.1150-1152.1995.
Abstract
A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2- to 5-ml) cultures. Moreover, this approach should be very useful in analytical detection and differentiation of Listeria strains when the release of native nucleic acids or proteins is required.
摘要
本文描述了一种利用重组李斯特菌噬菌体A118裂解酶（PLY118）快速裂解李斯特菌细胞的方法。该方法可用于所有李斯特菌属菌种。它能够从小规模（2至5毫升）培养物中快速、高效且温和地回收DNA、RNA或天然细胞蛋白。此外，当需要释放天然核酸或蛋白质时，这种方法在李斯特菌菌株的分析检测和鉴别中应非常有用。
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