Châtelain C, Pochon N, Lacroix J S
Department of Otorhinolaryngology, University Cantonal Hospital, Geneva, Switzerland.
Eur Arch Otorhinolaryngol. 1995;252(2):83-5. doi: 10.1007/BF00168025.
The functional effects of the intranasal application of exogenous vasoactive intestinal polypeptide (VIP), substance P (SP) and calcitonin gene-related peptide (CGRP) were evaluated in 12 healthy volunteers before and after neutral endopeptidase (NEP) inhibition with phosphoramidon (PA) and angiotensin-converting enzyme (ACE) inhibition with captopril. The three neuropeptides increased nasal airway resistance (NAR) measured by anterior rhinomanometry and superficial capillary blood flow measured by laser Doppler flowmetry (LDF). After pretreatment of the nasal mucosa with PA, the effects of VIP, SP and CGRP on the LDF signal, NAR and mucus production were potentiated, whereas local pretreatment with captopril did not modify these functional effects. These observations suggest that NEP, but not ACE, may participate in the catabolism of neuropeptides when applied directly to the human nasal mucosa. Furthermore, since these neuropeptides induced nasal obstruction, increased blood flow and rhinorrhea, a decreased activity of the enzymes involved in their degradation could be involved in the physiopathology of rhinitis symptoms.
在12名健康志愿者中，评估了用磷酰胺（PA）抑制中性内肽酶（NEP）和用卡托普利抑制血管紧张素转换酶（ACE）前后，经鼻应用外源性血管活性肠肽（VIP）、P物质（SP）和降钙素基因相关肽（CGRP）的功能效应。这三种神经肽增加了通过前鼻测压法测量的鼻气道阻力（NAR）以及通过激光多普勒血流仪（LDF）测量的浅表毛细血管血流量。在用PA对鼻黏膜进行预处理后，VIP、SP和CGRP对LDF信号、NAR和黏液分泌的作用增强，而用卡托普利进行局部预处理并未改变这些功能效应。这些观察结果表明，当直接应用于人体鼻黏膜时，NEP而非ACE可能参与神经肽的分解代谢。此外，由于这些神经肽会引起鼻塞、血流量增加和鼻溢，参与其降解的酶活性降低可能与鼻炎症状的病理生理过程有关。
