一种使用移液器吸头作为固相且使用pH敏感场效应晶体管作为检测器的自动化酶联免疫吸附测定（ELISA）系统。

An automated ELISA system using a pipette tip as a solid phase and a pH-sensitive field effect transistor as a detector.

作者信息

Tsuruta H, Yamada H, Motoyashiki Y, Oka K, Okada C, Nakamura M

机构信息

Central Research Laboratories, Kuraray Co. Ltd., Okayama, Japan.

出版信息

J Immunol Methods. 1995 Jun 28;183(2):221-9. doi: 10.1016/0022-1759(95)00058-i.

DOI:10.1016/0022-1759(95)00058-i

PMID:7541432

Abstract

A fully automated ELISA system was constructed using a pipette tip as a solid phase, urease as a detecting enzyme, and a pH-FET as a detector of urease activity. The inner wall of the end part of a pipette tip was used as a solid phase, and the urease activity of the conjugate, captured after a two-step immunoreaction, was measured by coupling the pipette tip with the pH-FET in a pH-measuring cell. Full automation of the ELISA system was achieved by using a disposable reagent cartridge and three pipetters for all mechanical operations, including sample dilution and B/F separation. This system can treat 60 samples per hour with an assay time of 21 min for all assay configurations. The system was applied to two-step sandwich assays for AFP, CEA, HBsAg, and HBsAb, a two-step competition assay for HBcAb, and a second antibody assay for HTLV-I Ab.

摘要

构建了一种全自动酶联免疫吸附测定（ELISA）系统，该系统使用移液器吸头作为固相，脲酶作为检测酶，pH场效应晶体管（pH-FET）作为脲酶活性的检测器。移液器吸头端部的内壁用作固相，在两步免疫反应后捕获的缀合物的脲酶活性，通过在pH测量池中使移液器吸头与pH-FET耦合来测量。ELISA系统的全自动化通过使用一次性试剂盒和三个移液器进行所有机械操作来实现，包括样品稀释和结合物与游离物（B/F）分离。该系统每小时可处理60个样品，所有检测配置的检测时间为21分钟。该系统应用于甲胎蛋白（AFP）、癌胚抗原（CEA）、乙型肝炎表面抗原（HBsAg）和乙型肝炎表面抗体（HBsAb）的两步夹心测定、乙型肝炎核心抗体（HBcAb）的两步竞争测定以及人类嗜T淋巴细胞病毒I型抗体（HTLV-I Ab）的二抗测定。