Conradie J D, Govender M, Visser L
J Immunol Methods. 1983 May 13;59(3):289-99. doi: 10.1016/0022-1759(83)90190-4.
We have examined conditions which will improve the solid phase in ELISAs for HBsAg and ferritin in human serum. Sheep and rabbit antibodies were used. It was found that pre-exposure of antibody to low pH, 3 M urea and temperatures as high as 82 degrees C will enhance ELISA colour in these assays. Further experiments indicate that these conditions perturb the structure of the antibody molecules and results in the exposure of new hydrophobic regions. It is speculated that these more hydrophobic molecules can bind to regions on the plastic surface normally not coated by non-perturbed molecules.
我们已经研究了能够改善人血清中乙肝表面抗原（HBsAg）和铁蛋白酶联免疫吸附测定（ELISA）固相的条件。使用了绵羊和兔抗体。结果发现，将抗体预先暴露于低pH值、3M尿素以及高达82摄氏度的温度下，会增强这些检测中的ELISA显色。进一步的实验表明，这些条件会扰乱抗体分子的结构，并导致新的疏水区域暴露。据推测，这些疏水性更强的分子可以结合到塑料表面上通常未被未受干扰分子包被的区域。
