Carr P A, Huang A, Noga B R, Jordan L M
Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Brain Res Bull. 1995;37(2):213-8. doi: 10.1016/0361-9230(94)00271-2.
Using standard immunohistochemical and histochemical techniques, we have examined the neurochemical characteristics of a subpopulation of locomotor-related neurons as labeled by the activity-dependent marker c-fos. Results were compared to those obtained from a small sample of intracellularly labeled locomotor-related neurons. In the paralyzed, decerebrate cat, fictive locomotion was evoked by electrical stimulation of the mesencephalic locomotor region. Most c-fos-immunoreactive neurons were distributed in medial lamina VI and VII and in lamina VIII and X. Double labeling of c-fos with various cytochemical markers revealed that about one-third of the c-fos-immunoreactive neurons were choline acetyltransferase immunoreactive, about one-third were glutamate immunoreactive, and about one-third were aspartate immunoreactive. In addition, approximately 15% of the c-fos-labeled neurons contained NADPH-diaphrorase reaction product, while almost 40% appeared to receive close contacts from calcitonin gene-related peptide-immunoreactive fibers and boutons. Choline acetyltransferase- or aspartate immunoreactivity was observed in some intracellularly labeled neurons. These findings have implications regarding the putative neurotransmitters utilized by subpopulations of locomotor-related neurons in the cat spinal cord.
运用标准免疫组织化学和组织化学技术，我们检测了由活性依赖标记物c-fos标记的与运动相关神经元亚群的神经化学特征。将结果与从一小部分细胞内标记的与运动相关神经元样本中获得的结果进行了比较。在瘫痪的去大脑猫中，通过电刺激中脑运动区诱发了虚构运动。大多数c-fos免疫反应性神经元分布在中间层VI和VII以及层VIII和X。用各种细胞化学标记物对c-fos进行双重标记显示，约三分之一的c-fos免疫反应性神经元为胆碱乙酰转移酶免疫反应性，约三分之一为谷氨酸免疫反应性，约三分之一为天冬氨酸免疫反应性。此外，约15%的c-fos标记神经元含有NADPH-黄递酶反应产物，而近40%的神经元似乎接受来自降钙素基因相关肽免疫反应性纤维和终扣的紧密接触。在一些细胞内标记的神经元中观察到胆碱乙酰转移酶或天冬氨酸免疫反应性。这些发现对猫脊髓中与运动相关神经元亚群所使用的假定神经递质具有启示意义。
