Charbord P, Caillou B, Lafleur M, Parmentier C
Nouv Rev Fr Hematol (1978). 1978 Nov 25;20(3):443-53.
Eight patients presenting primary myelofibrosis or spent polycythemia were submitted to a ferrokinetic study, a triphine bone marrow biopsy and a bone marrow puncture. In all cases before treatment (splenic radiotherapy) ferrokinetic measurements demonstrated an ineffective erythropoiesis, the intensity of which was precised on bone marrow biopsies, in numbering the erythroblasts per surface unit. Bone marrow biopsies permitted also, through a semiquantitative estimate of the bone marrow cellularity, to evaluate an aplasia component not always expected with ferrokinetic study. Study of bone marrow allowed to precise the site of the erythroblastic abortion; cellular death might occur during the last nitosis of the erythroblastic series (transition from the polychromatophil erythroblasts to the acidophil erythroblasts). In order to delineate the general pattern of erythropoiesis before treatment and when assessing the results of a treatment, besides ferrokinetic measurements, the study of bone marrow biopsy and smears appears relevant.
八名原发性骨髓纤维化或晚期真性红细胞增多症患者接受了铁动力学研究、三相骨髓活检和骨髓穿刺。在所有病例中，治疗前（脾放疗）铁动力学测量显示红细胞生成无效，其强度通过骨髓活检精确确定，即对每单位面积的成红细胞进行计数。骨髓活检还通过对骨髓细胞成分的半定量评估，得以评估铁动力学研究中并非总能预期到的再生障碍成分。骨髓研究能够精确成红细胞生成停滞的部位；细胞死亡可能发生在成红细胞系列的最后一次有丝分裂期间（从嗜多色性成红细胞向嗜酸性成红细胞转变）。为了描绘治疗前红细胞生成的总体模式以及评估治疗结果，除了铁动力学测量外，骨髓活检和涂片研究似乎也很重要。
