Malek-Hedayat S, Rome L H
Department of Biological Chemistry, UCLA School of Medicine 90024-1737, USA.
Gene. 1995 Jun 9;158(2):287-90. doi: 10.1016/0378-1119(94)00911-b.
We have isolated a cDNA clone encoding an integrin (Itg) beta-subunit polypeptide from a rat cerebral cortex oligodendroglia cDNA library. This beta-subunit was previously designated beta OL (OL for oligodendroglia), since it had an M(r) that differed from that of the rat fibroblast beta 1 protein. The complete nucleotide sequence encoding the signal sequence and mature protein was determined. Comparison of the deduced amino acid (aa) sequence of beta OL to those of integrin beta subunits from other species revealed that beta OL is a member of the integrin (Itg) beta 1 class. The aa sequence of beta OL shows 78.5-97.9% similarity to beta 1 chains from four other species and displays all the features characteristic of the Itg beta subunits, indicating the high degree of structural conservation seen in this subunit of the Itg receptors.
我们从大鼠大脑皮质少突胶质细胞cDNA文库中分离出一个编码整合素（Itg）β亚基多肽的cDNA克隆。该β亚基先前被命名为βOL（OL代表少突胶质细胞），因为其相对分子质量（M(r)）与大鼠成纤维细胞β1蛋白不同。确定了编码信号序列和成熟蛋白的完整核苷酸序列。将βOL推导的氨基酸（aa）序列与其他物种的整合素β亚基的序列进行比较，结果显示βOL是整合素（Itg）β1类的成员。βOL的aa序列与其他四个物种的β1链显示出78.5 - 97.9%的相似性，并具有整合素β亚基的所有特征，表明在整合素受体的该亚基中存在高度的结构保守性。
