Kobayashi H, O'Briain D S, Puri P
Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin, Ireland.
J Pediatr Gastroenterol Nutr. 1995 Apr;20(3):319-25. doi: 10.1097/00005176-199504000-00010.
The etiology of hypertrophic pyloric stenosis (HPS) is not known. We made an immunocytochemical examination of pyloric muscle from 18 patients with HPS and 10 controls using specific monoclonal antibodies to neural cell adhesion molecule (NCAM) as well as neurofilament protein and NADPH-diaphorase histochemistry. In HPS, bundles of hypertrophic muscle fibers expanded the circular muscle layer. The longitudinal muscle also appeared hypertrophic but to a less marked degree. The most striking difference between HPS and the control tissues was that NCAM, NADPH-diaphorase, and neurofilament protein immunoreactive fibers were absent or markedly reduced within the hypertrophied circular and longitudinal musculature. In contrast, NCAM, NADPH-diaphorase, and neurofilament protein immunoreactivity was preserved in the myenteric plexus where nerve fibers and ganglion cells were stained. The lack of expression of NCAM, NADPH-diaphorase, and neurofilament protein on nerve fibers within the circular and longitudinal muscle in patients with pyloric stenosis suggests that the smooth muscle is not innervated in this condition.
肥厚性幽门狭窄（HPS）的病因尚不清楚。我们使用针对神经细胞黏附分子（NCAM）的特异性单克隆抗体以及神经丝蛋白和NADPH - 黄递酶组织化学方法，对18例HPS患者和10例对照者的幽门肌进行了免疫细胞化学检查。在HPS中，肥厚的肌纤维束使环形肌层增厚。纵肌也呈现肥厚，但程度较轻。HPS与对照组织之间最显著的差异在于，在肥厚的环形和纵行肌组织中，NCAM、NADPH - 黄递酶和神经丝蛋白免疫反应性纤维缺失或明显减少。相比之下，在肌间神经丛中，神经纤维和神经节细胞被染色，NCAM、NADPH - 黄递酶和神经丝蛋白免疫反应性得以保留。幽门狭窄患者环形和纵行肌内神经纤维上NCAM、NADPH - 黄递酶和神经丝蛋白的表达缺失表明，在这种情况下平滑肌未受神经支配。
