Bogers J J, Timmermans J P, Scheuermann D W, Pelckmans P A, Mayer B, van Marck E A
Laboratory of Pathology, University of Antwerp (UIA), Belgium.
Ann Anat. 1994 Apr;176(2):131-5. doi: 10.1016/s0940-9602(11)80430-9.
Recent studies, using pharmacological or indirect morphological techniques, suggest that the non-adrenergic non-cholinergic (NANC) control of the ileocaecal junction (ICJ) is largely regulated by nitric oxide (NO). In this study, NO synthase (NOS) has been localized immunocytochemically and enzyme histochemically, using NADPH-diaphorase (NADPH-d), in enteric neurons of the myenteric and submucous plexuses of the ICJ of man and pig. The myenteric plexus, as well as the outer submucous plexus of both the porcine and the human ICJ, harboured NOS-containing neurons, which varied widely in size and shape, but which all displayed a multidendritic, uniaxonal appearance. Compared to the myenteric plexus, significantly fewer NOS-containing neurons were encountered in the outer submucous plexus. Neurofilament immunohistochemistry following NADPH-d application made it possible to distinguish a variety of cells that stained for both markers. Some of the larger neurons were of the Dogiel type-I morphology, whereas others showed a type III or a type VI-like morphology. A large number of NOS-immunoreactive nerve fibers were detected in the enlarged circular muscle of the ICJ and in the adjacent ileum. No NOS staining was detected in the smooth muscle cells of the outer circular or longitudinal muscle layer. The latter finding, together with the abundance of NOS positive nerve fibers in the smooth muscle layer, suggest a neuronal origin for NO as an important inhibitory neurotransmitter in the ICJ.
最近利用药理学或间接形态学技术进行的研究表明，回盲肠连接部（ICJ）的非肾上腺素能非胆碱能（NANC）控制很大程度上受一氧化氮（NO）调节。在本研究中，已使用烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d），通过免疫细胞化学和酶组织化学方法，对人和猪ICJ的肌间神经丛和黏膜下神经丛的肠神经元中的一氧化氮合酶（NOS）进行了定位。猪和人ICJ的肌间神经丛以及外层黏膜下神经丛都含有含NOS的神经元，这些神经元大小和形状差异很大，但均呈现多树突、单轴突外观。与肌间神经丛相比，外层黏膜下神经丛中含NOS的神经元明显较少。应用NADPH-d后进行神经丝免疫组织化学检测，能够区分出两种标记物均染色的多种细胞。一些较大的神经元具有Dogiel I型形态，而其他神经元则呈现III型或VI型样形态。在ICJ的增厚环形肌和相邻回肠中检测到大量NOS免疫反应性神经纤维。在外层环形或纵行肌层的平滑肌细胞中未检测到NOS染色。后一发现，连同平滑肌层中丰富的NOS阳性神经纤维，提示NO作为ICJ中一种重要的抑制性神经递质来源于神经元。
