Yamada H, Fujimoto S
Department of Obstetrics and Gynecology, School of Medicine, Hokkaido University, Sapporo, Japan.
Arch Gynecol Obstet. 1995;256(2):57-61. doi: 10.1007/BF00634709.
A kit using mouse monoclonal antibody against human alpha-fetoprotein (anti-AFP kit) has been developed. Cervical secretion (n = 34) and vaginal fluid (n = 15) was obtained from 30 pregnant volunteers. All 31 specimens obtained from women with intact membranes tested negative while all 18 specimens from women with premature rupture of membranes (PROM) tested positive. The newly developed kit allowed rapid and accurate diagnosis. We compared the kit results with those obtained by pH determination (nitrazine, bromthymol blue test) and measurement of AFP concentrations by enzyme-linked immunoassay (EIA).
已开发出一种使用抗人甲胎蛋白的小鼠单克隆抗体的试剂盒（抗甲胎蛋白试剂盒）。从30名怀孕志愿者身上获取了宫颈分泌物（n = 34）和阴道液（n = 15）。从胎膜完整的女性身上获取的所有31份样本检测均为阴性，而从胎膜早破（PROM）女性身上获取的所有18份样本检测均为阳性。新开发的试剂盒能够实现快速准确的诊断。我们将试剂盒的检测结果与通过pH测定（硝嗪、溴麝香草酚蓝试验）以及酶联免疫吸附测定（EIA）法测量甲胎蛋白浓度所获得的结果进行了比较。
