Spessert R, Layes E, Schollmayer A, Reuss S, Vollrath L
Department of Anatomy, Johannes Gutenberg University, Mainz, Germany.
Biochem Biophys Res Commun. 1995 Jul 6;212(1):70-6. doi: 10.1006/bbrc.1995.1937.
To investigate whether the expression of neuronal isoform of nitric oxide synthase is photoneurally regulated, we examined the amount of the enzyme by means of Western Blot analysis under different environmental lighting conditions in two photoneuronally regulated tissues, the pineal gland and the suprachiasmatic nucleus of the rat. In the pineal gland nitric oxide synthase immunoreactivity was strikingly decreased when rats had been exposed for 8 days to light:dark 20:4 conditions or to constant light. The decline in nitric oxide synthase immunoreactivity following LL reversed after 4 days under light:dark 12:12. We conclude that in the rat pineal the amount of neuronal nitric oxide synthase is controlled by environmental lighting conditions. That it is not justified to extrapolate from the pineal to other photoneuronally regulated centers is illustrated by the present finding that the suprachiasmatic nucleus did not reveal changes in the amount of neuronal nitric oxide synthase.
为了研究一氧化氮合酶神经元亚型的表达是否受光神经调节，我们在大鼠的两个受光神经调节的组织——松果体和视交叉上核中，通过蛋白质免疫印迹分析，在不同的环境光照条件下检测了该酶的含量。当大鼠暴露于光照:黑暗比例为20:4的条件或持续光照8天时，松果体中的一氧化氮合酶免疫反应性显著降低。在持续光照后，一氧化氮合酶免疫反应性的下降在光照:黑暗比例为12:12的条件下4天后逆转。我们得出结论，在大鼠松果体中，神经元型一氧化氮合酶的含量受环境光照条件的控制。视交叉上核未显示神经元型一氧化氮合酶含量的变化，这一目前的发现表明，从松果体推断到其他受光神经调节的中枢是不合理的。
