Schenda J, Vollrath L
Institute of Anatomy, University of Mainz, Federal Republic of Germany.
J Neural Transm (Vienna). 1997;104(1):53-8. doi: 10.1007/BF01271293.
Extracellular multiple unit recordings were performed in isolated rat pineal glands to determine a possible effect of nitric oxide (NO) on the spontaneous electrical activity of pinealocytes. Spontaneously active cells forming clusters of 3-5 cells fell into two categories: more or less regularly firing clusters (REG, 64%) and irregularly discharging clusters with periodically repeated bursts (RHY, 36%). The NO-donor sodium nitroprusside (SNP) reduced the discharge rate of the great majority of REG clusters and of all the RHY clusters examined. Moreover, the burst activity of RHY clusters was abolished. These results could be completely reproduced by using another NO-donor, S-nitroso-N-acetyl-penicillamine (SNAP). The NO synthase inhibitor NMLA had no effect on REG and RHY clusters. The results show that spontaneous electrical activity is an intrinsic function of the rat pineal gland. NO can modulate the electrical activity affecting discharge rate and discharge pattern.
在离体大鼠松果体中进行细胞外多单位记录，以确定一氧化氮（NO）对松果体细胞自发电活动的可能影响。形成3 - 5个细胞簇的自发活动细胞分为两类：或多或少规则放电的簇（REG，64%）和具有周期性重复爆发的不规则放电簇（RHY，36%）。NO供体硝普钠（SNP）降低了绝大多数REG簇以及所有检测的RHY簇的放电率。此外，RHY簇的爆发活动被消除。使用另一种NO供体S - 亚硝基 - N - 乙酰青霉胺（SNAP）可完全重现这些结果。NO合酶抑制剂NMLA对REG和RHY簇没有影响。结果表明，自发电活动是大鼠松果体的固有功能。NO可以调节电活动，影响放电率和放电模式。
