2'-氟多聚核苷酸指导的逆转录酶反应。同源多聚核苷酸的作用。

2'-Fluoropolynucleotide-directed reverse transcriptase reactions. Effect of homologous polynucleotides.

作者信息

Juca M B, Aoyama H

机构信息

Departamento de Bioquimica, Universidade Estadual de Campinas, S. Paulo, Brasil.

出版信息

J Enzyme Inhib. 1995;8(4):261-5. doi: 10.3109/14756369509020133.

DOI:10.3109/14756369509020133

PMID:7542323

Abstract

Several homologous polynucleotides have been tested as inhibitors on the reactions catalyzed by avian myeloblastosis virus (AMV) reverse transcriptase, in the presence of polyribonucleotides and 2'-fluorinated polynucleotides as templates. Polynucleotides differentially inhibited the reactions catalyzed by reverse transcriptase in the presence of these synthetic templates. Polyriboadenylic acid (poly(rA), poly(2'-O-methyladenylic acid) (poly(Am)), poly(2'-fluoro-2'-deoxyadenylic acid) (poly(dAfl), polyinosinic acid (poly(rI)) and polyuridylic acid poly(rU)) inhibited the polyribonucleotide-, but not the 2'-fluorinated polynucleotide-directed reverse transcriptase activity.

摘要

在以多聚核糖核苷酸和2'-氟化多聚核苷酸为模板的情况下，几种同源多聚核苷酸已被测试作为禽成髓细胞瘤病毒（AMV）逆转录酶催化反应的抑制剂。在这些合成模板存在的情况下，多聚核苷酸对逆转录酶催化的反应有不同程度的抑制作用。聚腺苷酸（poly(rA)）、聚（2'-O-甲基腺苷酸）（poly(Am)）、聚（2'-氟-2'-脱氧腺苷酸）（poly(dAfl)）、聚肌苷酸（poly(rI)）和聚尿苷酸（poly(rU)）抑制多聚核糖核苷酸指导的逆转录酶活性，但不抑制2'-氟化多聚核苷酸指导的逆转录酶活性。