各种2-和2'-取代聚腺苷酸对鼠白血病病毒逆转录酶的影响。

Influence of various 2- and 2'-substituted polyadenylic acids on murine leukemia virus reverse transcriptase.

作者信息

De Clercq E, Fukui T, Kakiuchi N, Ikehara M, Hattori M, Pfleiderer W

出版信息

Cancer Lett. 1979 Jun;7(1):27-37. doi: 10.1016/s0304-3835(79)80073-7.

Abstract

Several newly synthesized polyadenylic acid [(A)n] analogues, including poly(2-methyladenylic acid) [(m2A)n], poly(2-ethyladenylic acid) [(e2A)n], poly(2-isopropyladenylic acid) [(i-pro2A)n], poly(2-methylthioadenylic acid) [(ms2A)n], poly(2-ethylthioadenylic acid) [(e2A)n], poly(2'-fluoro-2'-deoxyadenylic acid) [(dAfl)n] and poly(2'-azido-2'-deoxyadenylic acid) [(dAz)n] have been evaluated for their effects on the RNA-directed DNA polymerase (reverse transcriptase) activity of Moloney murine leukemia virus; (m2A)n and (e2A)n did not markedly affect reverse transcriptase activity, (dAfl)n served as an efficient template for the reverse transcriptase reaction, and (i-pro2A)n, (ms2A)n, (es2A)n and (dAz)n strongly inhibited reverse transcriptase activity. (dAfl)n also served as an efficient template (Km : 0.025 micron) for the reverse transcriptase of avian myeloblastosis virus.

摘要

几种新合成的聚腺苷酸[（A）n]类似物，包括聚（2-甲基腺苷酸）[（m2A）n]、聚（2-乙基腺苷酸）[（e2A）n]、聚（2-异丙基腺苷酸）[（i-pro2A）n]、聚（2-甲硫基腺苷酸）[（ms2A）n]、聚（2-乙硫基腺苷酸）[（e2A）n]、聚（2'-氟-2'-脱氧腺苷酸）[（dAfl）n]和聚（2'-叠氮基-2'-脱氧腺苷酸）[（dAz）n]，已对它们对莫洛尼鼠白血病病毒的RNA指导的DNA聚合酶（逆转录酶）活性的影响进行了评估；（m2A）n和（e2A）n对逆转录酶活性没有明显影响，（dAfl）n是逆转录酶反应的有效模板，而（i-pro2A）n、（ms2A）n、（es2A）n和（dAz）n强烈抑制逆转录酶活性。（dAfl）n也是禽成髓细胞瘤病毒逆转录酶的有效模板（Km：0.025微米）。