精胺刺激RNA依赖性逆转录酶活性。
Spermine stimulates RNA-dependent reverse transcriptase activity.
作者信息
Aoyama H
机构信息
Departamento de Bioquímica, Universidade Estadual de Campinas, São Paulo, Brasil.
出版信息
Biochem Int. 1989 Jul;19(1):67-76.
The polyamine spermine can not replace Mg2+ in the reactions catalyzed by avian myeloblastosis virus reverse transcriptase. In the presence of suboptimal Mg2+ concentrations, spermine enhanced the reverse transcriptase activities with viral 70 S RNA and synthetic polyriboadenylic acid as templates, about 4-fold and 2-fold, respectively. A similar effect occurred in the polyriboadenylic acid-directed reaction using Mn2+ as divalent cation. On the other hand, no stimulation by spermine was observed with activated DNA and polydeoxyadenylic acid as template in the reactions catalyzed by reverse transcriptase.
在禽成髓细胞瘤病毒逆转录酶催化的反应中，多胺精胺不能替代Mg2+。在次优Mg2+浓度存在的情况下，精胺分别以病毒70S RNA和合成聚腺苷酸为模板，使逆转录酶活性提高了约4倍和2倍。在以Mn2+作为二价阳离子的聚腺苷酸指导的反应中也出现了类似的效果。另一方面，在逆转录酶催化的反应中，以活化DNA和聚脱氧腺苷酸为模板时，未观察到精胺的刺激作用。
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