Engel P, Zhou L J, Ord D C, Sato S, Koller B, Tedder T F
Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Immunity. 1995 Jul;3(1):39-50. doi: 10.1016/1074-7613(95)90157-4.
CD19-deficient mice were generated to examine the role of CD19 in B cell growth regulation in vivo. Deletion of CD19 had no deleterious effects on the generation of B cells in the bone marrow, but there was a significant reduction in the number of B cells in peripheral lymphoid tissues. B cells from CD19-deficient mice exhibited markedly decreased proliferative responses to mitogens, and serum immunoglobulin levels were also significantly decreased. In contrast, mice that overexpressed CD19 had significant defects in early B cell development in the bone marrow, augmented mitogenic responses, and increased serum immunoglobulin levels. These experiments indicate that CD19 functions to define signaling thresholds for cell surface receptors that regulate B lymphocyte selection, activation, and differentiation.
生成CD19缺陷小鼠以研究CD19在体内B细胞生长调节中的作用。CD19的缺失对骨髓中B细胞的生成没有有害影响，但外周淋巴组织中的B细胞数量显著减少。来自CD19缺陷小鼠的B细胞对有丝分裂原的增殖反应明显降低，血清免疫球蛋白水平也显著降低。相比之下，过表达CD19的小鼠在骨髓早期B细胞发育方面存在显著缺陷，有丝分裂原反应增强，血清免疫球蛋白水平升高。这些实验表明，CD19的功能是确定调节B淋巴细胞选择、激活和分化的细胞表面受体的信号阈值。
