Chung Y S, Yamada N, Sawada T, Takatsuka S, Sowa M
First Department of Surgery, Osaka City University, Medical School.
Nihon Rinsho. 1995 Jul;53(7):1747-52.
We first investigated the difference in carbohydrate antigens' expression between primary tumors and matched metastatic liver lesions of colorectal carcinomas, using immunohistochemical methods. Regarding the degree of expression, SLA, SLX and SPan-1 antigens were found to be expressed on a larger proportion of tumor cells in liver metastases than in primary tumors. Second, comparing the expression of carbohydrate antigens on highly metastatic colon cancer cell line to the parental line, the expression of SLA on the surface of highly metastatic variant was increased more intensively than the parental line. Finally, we investigated the contribution of carbohydrate antigens to endothelial E-selectin binding in colon and pancreatic cancer cells, and the results indicated that SLA might play a significant role in this binding. These findings suggest that the expression of carbohydrate antigens on the surface of cancer cells obviously contributed to E-selectin binding and also to metastatic potential.
我们首先采用免疫组化方法研究了结直肠癌原发肿瘤与配对的肝转移病灶中碳水化合物抗原表达的差异。关于表达程度，发现肝转移灶中比原发肿瘤中有更大比例的肿瘤细胞表达SLA、SLX和SPan-1抗原。其次，将高转移性结肠癌细胞系与亲代细胞系的碳水化合物抗原表达进行比较，高转移性变体表面SLA的表达比亲代细胞系增加得更强烈。最后，我们研究了碳水化合物抗原对结肠和胰腺癌细胞中内皮细胞E-选择素结合的作用，结果表明SLA可能在这种结合中起重要作用。这些发现提示癌细胞表面碳水化合物抗原的表达明显有助于E-选择素结合，也有助于转移潜能。
