人脐带血CD5⁺和CD5⁻ B淋巴细胞中免疫球蛋白重链互补决定区（CDR）-3的相似图谱。
Comparable profiles of the immunoglobulin heavy chain complementarity determining region (CDR)-3 in CD5+ and CD5- human cord blood B lymphocytes.
作者信息
Kiyoi H, Naito K, Ohno R, Naoe T
机构信息
Department of Medicine, Branch Hospital, Nagoya University School of Medicine, Japan.
出版信息
Immunology. 1995 Jun;85(2):236-40.
It has been suggested that CD5+ B cells are closely related to the pathogenesis of autoimmune disease or chronic lymphocytic leukaemia, whereas the origin and physiological role of CD5+ B cells remain controversial. To study the molecular differences between CD5+ and CD5- B cells in terms of immunoglobulin gene structure, we sorted both subsets from new-born cord blood and analysed the complementarity determining region (CDR)-3 profiles of the immunoglobulin heavy chain (IgH) gene. The CDR-3 sequences from both CD5+ and CD5- B cells represented the same incidence and length of N-region, and the same usage of D and JH segments. When translated into amino acids, 24 of 32 clones (75%) and 25 of 37 clones (65.8%) from their respective subset were productive, and the composition of the deduced amino acids were similar between both subsets. These data suggest that CD5+ B cells are not a distinct lineage presenting a biased immunoglobulin repertoire in B cells.
有人提出，CD5⁺ B细胞与自身免疫性疾病或慢性淋巴细胞白血病的发病机制密切相关，而CD5⁺ B细胞的起源和生理作用仍存在争议。为了从免疫球蛋白基因结构方面研究CD5⁺和CD5⁻ B细胞之间的分子差异，我们从新生儿脐带血中分离出这两个亚群，并分析了免疫球蛋白重链（IgH）基因的互补决定区（CDR）-3图谱。CD5⁺和CD5⁻ B细胞的CDR-3序列显示出相同的N区发生率和长度，以及相同的D和JH区段使用情况。当翻译成氨基酸时，各自亚群中32个克隆中的24个（75%）和37个克隆中的25个（65.8%）具有表达活性，并且两个亚群之间推导氨基酸的组成相似。这些数据表明，CD5⁺ B细胞不是一个在B细胞中呈现偏向性免疫球蛋白库的独特谱系。
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