Miller R A, Gralow J
J Immunol. 1984 Dec;133(6):3408-14.
Malignant cells from five patients with B cell leukemia or lymphoma were cultured with phorbol myristic acetate (PMA). PMA was found to induce cell surface expression of the Leu-1 antigen in cells from three of the five patients. Using one- and two-color immunofluorescence staining and flow cytometry, we have shown simultaneous expression of the Leu-1 antigen with other B cell markers. Induction of Leu-1 antigen expression was not related to histologic subtype, in vitro secretion of immunoglobulin, or other clinical features. Biosynthetic labeling experiments showed that synthesis of Leu-1 antigen occurred and preceded expression of the antigen on the cell surface. PMA also induced the appearance of Leu-1 antigen-positive B cells in cultures of normal peripheral blood mononuclear cells. We propose that the Leu-1 antigen is expressed transiently during the differentiation of some B cells.
将来自5例B细胞白血病或淋巴瘤患者的恶性细胞与佛波醇肉豆蔻酸酯（PMA）一起培养。发现PMA可诱导5例患者中3例患者的细胞表面表达Leu-1抗原。通过单克隆和双色免疫荧光染色及流式细胞术，我们已证明Leu-1抗原与其他B细胞标志物同时表达。Leu-1抗原表达的诱导与组织学亚型、免疫球蛋白的体外分泌或其他临床特征无关。生物合成标记实验表明，Leu-1抗原的合成发生在该抗原在细胞表面表达之前。PMA还可诱导正常外周血单个核细胞培养物中出现Leu-1抗原阳性B细胞。我们提出，Leu-1抗原在某些B细胞分化过程中短暂表达。
