Sriplung H, Kietthubthew S, Boonyaphiphat P
Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Thailand.
J Med Assoc Thai. 1995 Mar;78(3):140-4.
A polychrome method for detection of mucin substance in paraffin section is produced by sequential stepwise staining of hematoxylin, crude lac extract (Laccifer lacca), and crude curcuma extract (khamin shan-Curcuma longa). The name LacCur stain is proposed. After a tissue section is deparaffinized and rehydrated, it is stained with Weigert's hematoxylin for 7 minutes. After a quick wash, it is stained for at least 3 hours with lac dye mordanted with aluminum chloride. Washed again and premordanted with ferric chloride for 1 minute, in the last step, it is counterstained with curcuma dye for 5 minutes. With this staining method, the nuclei are stained black, mucin deep red, and organelles and ground substances brownish yellow. The method and outcome colors are comparable to the widely used Mayer's mucicarmine staining method. It costs less than the Mayer's mucicarmine staining method and the procedure is not complicated.
一种用于检测石蜡切片中黏蛋白物质的多色方法，是通过苏木精、粗紫胶提取物（紫胶虫）和粗姜黄提取物（山姜黄-姜黄）的顺序逐步染色来实现的。我们提出了LacCur染色法这一名称。组织切片脱蜡复水后，用魏格特苏木精染色7分钟。快速冲洗后，用经氯化铝媒染的紫胶染料染色至少3小时。再次冲洗并用氯化铁预媒染1分钟，最后一步用姜黄染料复染5分钟。用这种染色方法，细胞核染成黑色，黏蛋白染成深红色，细胞器和基质染成棕黄色。该方法及其染色结果与广泛使用的迈尔黏蛋白胭脂红染色法相当。它的成本低于迈尔黏蛋白胭脂红染色法，且操作不复杂。
