从骨髓和外周血干细胞中通过介电泳分离和富集CD34+细胞亚群。
Dielectrophoretic separation and enrichment of CD34+ cell subpopulation from bone marrow and peripheral blood stem cells.
作者信息
Talary M S, Mills K I, Hoy T, Burnett A K, Pethig R
机构信息
Institute of Molecular and Biomolecular Electronics, University of Wales, Gwynedd, UK.
出版信息
Med Biol Eng Comput. 1995 Mar;33(2):235-7. doi: 10.1007/BF02523050.
PMID:7543968
Abstract
Dielectrophoresis has been used to enrich selected cell subpopulations in a mixed cell population by exploiting differential dielectric properties. Six-fold enrichment of stem cells expressing the CD34+ antigen has been achieved for bone marrow samples and peripheral blood, without the requirement for initial chemical treatment associated with immunoadsorption techniques.
摘要
介电电泳已被用于通过利用不同的介电特性来富集混合细胞群体中选定的细胞亚群。对于骨髓样本和外周血，已实现表达CD34+抗原的干细胞六倍富集，而无需与免疫吸附技术相关的初始化学处理。
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