Markx G H, Talary M S, Pethig R
Institute of Molecular and Biomolecular Electronics, University of Wales, Bangor, Gwynedd, UK.
J Biotechnol. 1994 Jan 15;32(1):29-37. doi: 10.1016/0168-1656(94)90117-1.
Dielectrophoresis, the movement of particles in non-uniform AC electric fields, was used to rapidly separate viable and non-viable yeast cells with good efficiency. Known mixtures of viable and heat-treated cells of Saccharomyces cerevisiae were separated and selectively isolated using positive and negative dielectrophoretic forces generated by microelectrodes in a small chamber. Good correlations with the initial known relative compositions were obtained by direct microscopic counting of cells at the electrodes after initial dielectrophoretic separation (r = 0.995), from methylene blue staining (r = 0.992) and by optical absorption measurements (r = 0.980) of the effluent after selectively flushing out the viable and non-viable cells from the chamber. Through measurement of cell viability by staining with methylene blue and plate counts, for an initial suspension of approx. 1.4 x 10(7) cells per ml containing 60% non-viable cells, the dielectrophoretically separated non-viable fraction contained 3% viable cells and the viable fraction 8% dead cells. The separation efficiency is increased by dilution of the initial suspension or by repeat operation(s). Cell viability was not affected by the separation procedure.
介电泳，即颗粒在非均匀交流电场中的移动，被用于高效快速地分离活酵母细胞和非活酵母细胞。使用小腔室中的微电极产生的正介电泳力和负介电泳力，对酿酒酵母的活细胞和热处理细胞的已知混合物进行了分离和选择性分离。在初始介电泳分离后，通过对电极处的细胞进行直接显微镜计数（r = 0.995）、亚甲基蓝染色（r = 0.992）以及对从小腔室中选择性冲洗出活细胞和非活细胞后的流出物进行光吸收测量（r = 0.980），得到了与初始已知相对组成的良好相关性。通过用亚甲基蓝染色和平板计数来测量细胞活力，对于每毫升约含1.4×10⁷个细胞且60%为非活细胞的初始悬浮液，介电泳分离出的非活部分含有3%的活细胞，活部分含有8%的死细胞。通过稀释初始悬浮液或重复操作可提高分离效率。细胞活力不受分离过程的影响。
