Gascoyne P R, Pethig R, Burt J P, Becker F F
Department of Molecular Pathology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Biochim Biophys Acta. 1993 Jun 18;1149(1):119-26. doi: 10.1016/0005-2736(93)90032-u.
Dielectrophoresis measurements obtained using an image processing technique are reported over the frequency range 1 Hz to 100 kHz for the Friend murine erythroleukemia cell lines DS19 and R1 before and after treatment with hexamethylene bisacetamide and dimethylsulfoxide, agents that induce terminal differentiation in DS19 but not in R1 cells. Data are analyzed according to the single shell dielectric model of the cell. The membrane capacitance was found to fall by 30% and membrane conductivity by a factor of at least 5 when DS19 cells were induced to differentiate. R1 cells showed no such response. While the theoretical model was found to be useful for comparing differences in data for the different cell lines, several significant discrepancies between its predictions and the experimental data were observed, including positive dielectrophoretic collection at frequencies below 20 Hz and a smaller than predicted response to the membrane permeabilizing agents saponin and valinomycin. Factors that may have accounted for these discrepancies include surface charge effecgs, conduction parallel to the plasma membrane surface, and intracellular compartments.
报告了使用图像处理技术在1 Hz至100 kHz频率范围内对Friend小鼠红白血病细胞系DS19和R1进行介电电泳测量的结果，测量是在细胞用六亚甲基双乙酰胺和二甲基亚砜处理之前和之后进行的，这两种试剂可诱导DS19细胞终末分化，但不能诱导R1细胞分化。根据细胞的单壳介电模型对数据进行分析。当DS19细胞被诱导分化时，发现其膜电容下降了30%，膜电导率至少下降了5倍。R1细胞没有这种反应。虽然发现理论模型有助于比较不同细胞系数据的差异，但观察到其预测与实验数据之间存在一些显著差异，包括在低于20 Hz的频率下出现正介电电泳收集，以及对膜通透剂皂角苷和缬氨霉素的反应小于预测值。可能导致这些差异的因素包括表面电荷效应、平行于质膜表面的传导以及细胞内区室。
