Satoh K, Arai R, Ikemoto K, Narita M, Nagai T, Ohshima H, Kitahama K
Department of Psychiatry, Shiga University of Medical Science, Otsu, Japan.
Neuroscience. 1995 Jun;66(3):685-96. doi: 10.1016/0306-4522(95)00040-p.
The distribution of nitric oxide synthase-immunoreactive neurons was studied in the Macaca fuscata by immunohistochemistry using antiserum against nitric oxide synthase. In the macaque lower brainstem, many nitric oxide synthase-containing cell bodies were found in the gigantocellular and parvocellular reticular nuclei, the nucleus of the spinal tract of trigeminal nerve, the cochlear nucleus, the prepositus hypoglossi and the nucleus of the solitary tract. Many nitric oxide synthase-immunoreactive perikarya were observed in the laterodorsal-pedunculopontine tegmental nucleus complex of the macaque pontine and midbrain tegmentum. In addition, there were many highly immunoreactive cell bodies in the superficial layers of the inferior and superior colliculi. In the forebrain, numerous cell bodies were observed in the caudate nucleus, putamen, nucleus accumbens, nucleus of the diagonal band, anterior perforated substance and amygdaloid complex. Whereas most of these neurons were labeled highly intense for nitric oxide synthase, there were also many lightly labeled nitric oxide synthase-immunoreactive neurons in the substantia innominata, globus pallidus, ansa peduncularis and lateral hypothalamic nucleus. The present observation indicated some species difference in the distribution of central nitric oxide synthase-containing neurons. Furthermore, the present neuroanatomical evidence that nitric oxide synthase is distributed in a variety of specific neuronal systems, with some differences in the patterns of cytoplasmic localization, further indicates the neural messenger role of nitric oxide in the central nervous system.
利用抗一氧化氮合酶抗血清，通过免疫组织化学方法研究了猕猴中一氧化氮合酶免疫反应性神经元的分布。在猕猴脑干下部，在巨细胞网状核和小细胞网状核、三叉神经脊束核、蜗神经核、舌下前置核和孤束核中发现了许多含一氧化氮合酶的细胞体。在猕猴脑桥和中脑被盖的背外侧脚桥被盖核复合体中观察到许多一氧化氮合酶免疫反应性核周体。此外，在上下丘的表层有许多高免疫反应性细胞体。在前脑，在尾状核、壳核、伏隔核、斜角带核、前穿质和杏仁复合体中观察到许多细胞体。虽然这些神经元中的大多数对一氧化氮合酶标记强烈，但在无名质、苍白球、脚间袢和下丘脑外侧核中也有许多轻度标记的一氧化氮合酶免疫反应性神经元。本观察结果表明，含中枢一氧化氮合酶的神经元分布存在一些种属差异。此外，目前关于一氧化氮合酶分布于多种特定神经元系统且细胞质定位模式存在一些差异的神经解剖学证据，进一步表明了一氧化氮在中枢神经系统中的神经信使作用。
