White J L, Tousignant M E, Geletka L M, Kaper J M
Molecular Plant Pathology Laboratory, U. S. Department of Agriculture, Beltsville, Maryland, USA.
Arch Virol. 1995;140(1):53-63. doi: 10.1007/BF01309723.
Lethal necrosis development in tomato plants infected with cucumber mosaic virus (CMV) strain D containing the necrogenic satellite D-CARNA 5 and held at 32 degrees C is shown to be impaired. CARNA 5 accumulation in tomato at 32 degrees C is reduced about 100-fold compared to accumulation in plants held at 24 degrees C, while viral RNA accumulation is reduced about 5-fold. CMV-infected tomato held for 3 days at 24 degrees C prior to shift to 32 degrees C do not develop lethal necrosis. Longer incubations at 24 degrees C prior to shift to 32 degrees C allow necrosis to develop. CMV-infected plants held for up to 4 weeks at 32 degrees C required an additional 8-10 days at 24 degrees C to develop necrosis. Necrogenic CMV-infected plants held at 24 degrees C and analyzed 3 days p.i. contained detectable amounts of ss- and ds-CARNA 5; upon shift to 32 degrees C, such CARNA 5 declined to undetectable levels and lethal necrosis did not occur. There appear to be temperature-sensitive factors that are required for efficient satellite replication which are not required for efficient viral RNA replication. Whether these factor(s) are of host or satellite origin is uncertain.
研究表明，感染了含有坏死性卫星D - CARNA 5的黄瓜花叶病毒（CMV）株系D并置于32摄氏度环境下的番茄植株，其致死性坏死的发展受到了抑制。与置于24摄氏度环境下的植株相比，32摄氏度时番茄中CARNA 5的积累量减少了约100倍，而病毒RNA的积累量减少了约5倍。在转移至32摄氏度之前，先在24摄氏度下放置3天的CMV感染番茄不会出现致死性坏死。在转移至32摄氏度之前，在24摄氏度下培养更长时间会使坏死发生。在32摄氏度下放置长达4周的CMV感染植株，需要在24摄氏度下再放置8 - 10天才能出现坏死。在24摄氏度下培养并在接种后3天进行分析的坏死性CMV感染植株含有可检测到的单链和双链CARNA 5；转移至32摄氏度后，这种CARNA 5降至无法检测的水平，且未发生致死性坏死。似乎存在高效卫星复制所需的温度敏感因子，而高效病毒RNA复制则不需要这些因子。这些因子是宿主来源还是卫星来源尚不确定。
