Kaper J M, Geletka L M, Wu G S, Tousignant M E
Molecular Plant Pathology Laboratory, U.S. Department of Agriculture, Beltsville, Maryland, USA.
Arch Virol. 1995;140(1):65-74. doi: 10.1007/BF01309724.
The effect of temperature on the response of tomato (Lycopersicon esculentum Mill. cv. Rutgers) to infections with the necrogenic cucumber mosaic virus (CMV) satellite D-CARNA 5 was investigated with each of four CMV strains D, 1, Y and S functioning as helper virus. At 24 degrees C lethal necrosis was observed in all infections. However, at 32 degrees C the response varied from total absence or reduction of necrosis with some strains to accelerated lethal necrosis with others. The total lack of necrotic response with CMV-S and the aggravated necrosis with CMV-Y at the higher temperature both turned out to be independent of the coinfecting satellite, and rather to correlate with the changing rate of viral RNA accumulation in tomato, which probably was responsible for the changes in pathogenic response. However, when CMV-D was helper virus, satellite accumulation decreased, while with CMV-1 it increased, respectively, while viral RNA accumulations were not seriously affected. Although these profound effects of temperature seem to link the necrotic response of tomato to the competitive replication dynamics of the infecting virus/satellite combination in the case of CMV-D/D-CARNA 5, temperature effects at other levels of disease induction probably play an important role as well.
以四种黄瓜花叶病毒（CMV）株系D、1、Y和S作为辅助病毒，研究了温度对番茄（Lycopersicon esculentum Mill. cv. Rutgers）感染坏死性黄瓜花叶病毒卫星D-CARNA 5的反应的影响。在24℃时，所有感染中均观察到致死性坏死。然而，在32℃时，反应有所不同，一些株系导致坏死完全缺失或减少，而另一些株系则导致致死性坏死加速。在较高温度下，CMV-S完全没有坏死反应，CMV-Y导致坏死加剧，这两者都与共感染的卫星无关，而是与番茄中病毒RNA积累的变化速率相关，这可能是致病反应变化的原因。然而，当CMV-D作为辅助病毒时，卫星积累减少，而当CMV-1作为辅助病毒时，卫星积累增加，而病毒RNA积累没有受到严重影响。尽管温度的这些深远影响似乎在CMV-D/D-CARNA 5的情况下将番茄的坏死反应与感染病毒/卫星组合的竞争性复制动态联系起来，但温度在疾病诱导的其他层面的影响可能也起着重要作用。
