van der Flier A, Kuikman I, Baudoin C, van der Neut R, Sonnenberg A
The Netherlands Cancer Institute, Division of Cell Biology, Amsterdam, The Netherlands.
FEBS Lett. 1995 Aug 7;369(2-3):340-4. doi: 10.1016/0014-5793(95)00814-p.
The mRNA's of several integrin subunits are alternatively spliced in the region encoding cytoplasmic domains, that may potentially provide alternative integrin-cytoskeleton interactions and transmembrane signaling pathways. We identified a novel cytoplasmic tail variant of the human beta 1 subunit by reverse transcriptase polymerase chain reaction. This fourth beta 1 variant, named beta 1D, is specific for skeletal and cardiac muscle. The determined genomic organization of the 3'-region of the human beta 1 gene reveals that beta 1D is produced by alternative splicing of mRNA. In addition, we show that the expression of beta 1D is developmentally regulated during murine myoblast differentiation, suggesting a role for beta 1D in myogenesis.
几种整合素亚基的信使核糖核酸（mRNA）在编码细胞质结构域的区域进行可变剪接，这可能潜在地提供了替代的整合素-细胞骨架相互作用和跨膜信号通路。我们通过逆转录聚合酶链反应鉴定出一种人类β1亚基的新型细胞质尾部变体。这种第四个β1变体，命名为β1D，对骨骼肌和心肌具有特异性。所确定的人类β1基因3'区域的基因组结构表明，β1D是由mRNA的可变剪接产生的。此外，我们表明β1D的表达在小鼠成肌细胞分化过程中受到发育调控，这表明β1D在肌生成中发挥作用。
