Tao Y X, Lei Z M, Woodworth S H, Rao C V
Department of Obstetrics and Gynecology, University of Louisville School of Medicine, KY 40292, USA.
Mol Cell Endocrinol. 1995 Apr 28;111(1):R9-12. doi: 10.1016/0303-7207(95)03564-n.
The reverse transcription-polymerase chain reaction (RT-PCR) amplified an expected 255 bp luteinizing hormone/chorionic gonadotropin (LH/CG) receptor sequence from rat prostates. Northern blotting demonstrated that prostates contain 4.3, 3.3, 2.6, 1.8, 0.8 and 0.2 kb LH/CG receptor transcripts. Western immunoblotting and ligand blotting demonstrated that prostates also contain an 80 kDa receptor protein which can bind 125I-labeled hCG and this binding was inhibited by excess unlabeled hCG. In situ hybridization and immunocytochemistry revealed that while the receptors are most abundant in epithelial cells, they are scarcely found in the stroma. The ventral lobe contained more receptors than the lateral lobe and the receptors in peripheral acini of the ventral lobe are higher which progressively decreased towards central acini. In summary, prostate glands express LH/CG receptor gene. The cellular, topographical and lobular distribution of receptors suggest that LH may directly regulate prostate functions.
逆转录聚合酶链反应（RT-PCR）从大鼠前列腺中扩增出预期的255 bp促黄体生成素/绒毛膜促性腺激素（LH/CG）受体序列。Northern印迹法表明前列腺含有4.3、3.3、2.6、1.8、0.8和0.2 kb的LH/CG受体转录本。Western免疫印迹法和配体印迹法表明前列腺还含有一种80 kDa的受体蛋白，它能结合125I标记的hCG，且这种结合被过量的未标记hCG抑制。原位杂交和免疫细胞化学显示，虽然受体在上皮细胞中含量最多，但在基质中几乎找不到。腹叶比侧叶含有更多的受体，腹叶外周腺泡中的受体更高，向中央腺泡逐渐减少。总之，前列腺表达LH/CG受体基因。受体的细胞、拓扑和小叶分布表明LH可能直接调节前列腺功能。
