Yuki N, Handa S, Tai T, Takahashi M, Saito K, Tsujino Y, Taki T
Department of Biochemistry, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
J Neurol Sci. 1995 May;130(1):112-6. doi: 10.1016/0022-510x(95)00045-4.
Sera from patients with Guillain-Barré syndrome (GBS) frequently have anti-GM1 antibody. We earlier showed that an lipopolysaccharides (LPS) from Campylobacter jejuni (PEN 19) isolated from a GBS patient has a GM1 ganglioside-like structure. Aspinall et al. (Biochemistry, 61 (1994) 335-337) reported that OH 4382 has an LPS that bears a CD3 ganglioside-like structure and that OH 4384 has an LPS that bears a GT1a-like structure; both strains were isolated from patients with GBS. They also suggested a GM1-like structure is present in the LPSs from OH 4384, but failed to show the presence in the LPSs from OH 4382. To clarify the pathogenesis of GBS after infection by C. jejuni (PEN 19), we investigated the carbohydrate structures of the three strains by thin-layer chromatography immunostaining with cholera toxin and monoclonal anti-ganglioside antibodies. We found that both OH 4382 and OH 4384 have an LPS with the GM1 epitope as well as one with the GT1a or GD3 epitope.
吉兰-巴雷综合征（GBS）患者的血清中经常存在抗GM1抗体。我们之前表明，从一名GBS患者分离出的空肠弯曲菌（PEN 19）的脂多糖（LPS）具有GM1神经节苷脂样结构。阿斯皮诺尔等人（《生物化学》，61（1994）335 - 337）报告称，OH 4382的LPS具有CD3神经节苷脂样结构，OH 4384的LPS具有GT1a样结构；这两种菌株均从GBS患者中分离得到。他们还提出OH 4384的LPS中存在GM1样结构，但未能证明OH 4382的LPS中存在该结构。为了阐明空肠弯曲菌（PEN 19）感染后GBS的发病机制，我们通过用霍乱毒素和单克隆抗神经节苷脂抗体进行薄层色谱免疫染色，研究了这三种菌株的碳水化合物结构。我们发现OH 4382和OH 4384的LPS均具有GM1表位以及GT1a或GD3表位。
