Dombrádi V, Vereb G
Acta Biochim Biophys Acad Sci Hung. 1978;13(1-2):7-12.
The reaction of phosphorylases from rabbit skeletal muscle, pig skeletal muscle and pig heart with DTNB and the effects of AMP and glucose-6-phosphate on the above reaction were examined and compared. The SH groups of pig heart-specific phosphorylase b were found to be the same as those of rabbit skeletal muscle. Pig skeletal muscle phosphorylase b differed from the foregoing enzymes in that its slowly reacting SH groups played a minor role in the catalytic activity and AMP and glucose-6-phosphate affected neither the DTNB reaction nor the small decrease of activity during the reaction.
研究并比较了兔骨骼肌、猪骨骼肌和猪心脏中的磷酸化酶与5,5'-二硫代双(2-硝基苯甲酸)(DTNB)的反应，以及AMP和6-磷酸葡萄糖对上述反应的影响。发现猪心脏特异性磷酸化酶b的巯基与兔骨骼肌的巯基相同。猪骨骼肌磷酸化酶b与上述酶不同，其反应缓慢的巯基在催化活性中起次要作用，AMP和6-磷酸葡萄糖既不影响DTNB反应，也不影响反应过程中活性的微小降低。
