Hesselbarth J, Schwarz S
Institut für Kleintierforschung Cell/Merbitz der Bundesforschungsanstalt für Landwirtschaft Braunschweig-Völkenrode (FAL), Germany.
Vet Microbiol. 1995 Jun;45(1):11-7. doi: 10.1016/0378-1135(94)00125-g.
Strains of Staphylococcus (S.) intermedius from dogs, pigeons, horses and mink were typed by comparison of rRNA gene restriction fragment length polymorphisms (ribotyping) and the resulting ribotypes examined by cluster-analysis. Digestion of whole-cell DNA with HindIII resulted in 9 ribotypes with 3 to 4 bands. Separation of isolates from different host animal species was not possible. EcoRI yielded 11 different patterns with 4 to 9 fragments. The EcoRI-ribotypes of all canine strains grouped in one cluster encompassing four closely related ribotypes. Isolates were indistinguishable with respect to their origin from cases of canine pyoderma or from healthy carriers. In contrast, pigeon and equine strains expressed variety of ribotypes including some strains with patterns similar to canine isolates. However, for canine versus pigeon and equine strains the difference in distribution among ribotypes was significant (P < 0.001). The data might suggest that pigeons and horses carry S. intermedius transiently and might be able to spread these bacteria to other animal species.
通过比较rRNA基因限制性片段长度多态性（核糖体分型）对来自犬、鸽、马和水貂的中间葡萄球菌菌株进行分型，并通过聚类分析检查所得的核糖体分型。用HindIII消化全细胞DNA产生了9种核糖体分型，有3至4条带。无法区分来自不同宿主动物物种的分离株。EcoRI产生了11种不同的模式，有4至9个片段。所有犬类菌株的EcoRI核糖体分型聚集在一个包含四种密切相关核糖体分型的簇中。分离株在起源上无法区分是来自犬脓皮病病例还是健康携带者。相比之下，鸽和马的菌株表现出多种核糖体分型，包括一些与犬分离株模式相似的菌株。然而，对于犬类与鸽类和马类菌株，核糖体分型之间的分布差异是显著的（P < 0.001）。数据可能表明鸽和马短暂携带中间葡萄球菌，并可能能够将这些细菌传播到其他动物物种。
