Hesselbarth J, Witte W, Cuny C, Rohde R, Amtsberg G
Department of Microbiology and Infectious Diseases, School of Veterinary Medicine, Hannover, Germany.
Vet Microbiol. 1994 Aug 1;41(3):259-66. doi: 10.1016/0378-1135(94)90106-6.
In order to study the possible clonal relation of Staphyloccocus (S.) intermedius from canine superficial pyoderma and from healthy carriers, isolates from pustular swabs and from vaginal, nasal and normal skin sabs were typed using macrorestriction analysis with Sma I and pulsed-field gel electrophoresis. From the size of the resulting fragments the size of the chromosome of S. intermedius could be determined to be roughly 1500 +/- 200 kb on the average. The fingerprints were very heterogeneous though characteristically distinct from patterns of (human) S. aureus as published by others. Strains from superficial pyoderma were not found to be more similar to each other than strains from healthy carriers. Therefore it was concluded that strains from this type of skin infection probably did not belong to a certain subpopulation of S. intermedius, which might have indicated a higher virulence of these strains.
为了研究犬浅表脓疱性皮炎分离株与健康带菌者中间葡萄球菌之间可能存在的克隆关系，对脓疱拭子、阴道、鼻腔及正常皮肤拭子分离的菌株，采用Sma I酶切宏观限制性分析和脉冲场凝胶电泳进行分型。根据所得片段的大小，可确定中间葡萄球菌染色体的平均大小约为1500 +/- 200 kb。尽管指纹图谱与其他人发表的（人）金黄色葡萄球菌的图谱有明显差异，但非常不均一。未发现浅表脓疱性皮炎分离株之间比健康带菌者分离株之间更为相似。因此得出结论，这种皮肤感染分离株可能不属于中间葡萄球菌的某个特定亚群，否则可能表明这些菌株具有更高的毒力。
