Pálos L A, Blaskó G, Machovich R
Acta Physiol Acad Sci Hung. 1977;49(2):95-101.
The effect of some mono- and divalent cations was examined on thrombin--antithrombin reaction in vitro. It was found that 0--0.1 M sodium- or potassium chloride did not affect either thrombin or antithrombin activity; at higher concentrations thrombin activity decreased. Calcium chloride as well as magnesium chloride at concentrations from 0 to 0.5 M increased enzyme activity, whereas at higher concentrations the activity decreased. Thrombin inactivation by antithrombin was also accelerated at calcium or magnesium chloride concentrations above 0.04 M. Antithrombin was inactivated at pH 7.3 at 65 degrees C in some minutes and heparin failed to protect it against heat denaturation. Thrombin inactivation by antithrombin did not proceed at 0 degrees C in 60 min, but the interaction between thrombin and antithrombin was facilitated in the presence of heparin.
研究了一些单价和二价阳离子对凝血酶-抗凝血酶体外反应的影响。发现0-0.1M的氯化钠或氯化钾对凝血酶或抗凝血酶活性均无影响；浓度较高时，凝血酶活性降低。浓度为0至0.5M的氯化钙和氯化镁均能增加酶活性，而浓度更高时活性则降低。当氯化钙或氯化镁浓度高于0.04M时，抗凝血酶对凝血酶的灭活作用也会加速。抗凝血酶在pH7.3、65℃条件下几分钟内就会失活，肝素不能保护其免受热变性影响。抗凝血酶在0℃条件下60分钟内不会使凝血酶失活，但在肝素存在的情况下，凝血酶与抗凝血酶之间的相互作用会得到促进。
