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与人类神经生长因子结合的RNA配体。

RNA ligands to human nerve growth factor.

作者信息

Binkley J, Allen P, Brown D M, Green L, Tuerk C, Gold L

机构信息

Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347, USA.

出版信息

Nucleic Acids Res. 1995 Aug 25;23(16):3198-205. doi: 10.1093/nar/23.16.3198.

DOI:10.1093/nar/23.16.3198
PMID:7545283
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307178/
Abstract

High affinity RNA ligands to human nerve growth factor (NGF) were selected from pools of random RNA using SELEX [Tuerk, C. and Gold, L. (1990) Science, 249, 505-510]. Nerve growth factor, which is a protein required for the development of neurons, is not known to bind nucleic acids as part of its natural function. We describe two of the selected RNA molecules in detail. One of them is highly structured, folding into a pseudoknot with an additional hairpin-loop; this structure provides salt-resistant binding to NGF. The other is unstructured and elevated salt concentrations inhibit its binding. These molecules compete with each other for NGF binding. Our RNAs may furnish useful diagnostic tools for the study of an important neurotrophic protein; additionally, they illustrate another example of the potential for nucleic acids to take part in novel binding interactions.

摘要

利用指数富集的配体系统进化技术（SELEX）[图尔克，C.和戈尔德，L.（1990年）《科学》，249卷，505 - 510页]从随机RNA文库中筛选出了与人神经生长因子（NGF）具有高亲和力的RNA配体。神经生长因子是神经元发育所需的一种蛋白质，目前尚未发现其天然功能中存在与核酸结合的情况。我们详细描述了其中两个筛选出的RNA分子。其中一个具有高度结构化，折叠成带有一个额外发夹环的假结结构；这种结构赋予了其对NGF的耐盐结合能力。另一个则是无结构的，高盐浓度会抑制其与NGF的结合。这些分子在与NGF的结合上相互竞争。我们筛选出的这些RNA可能为研究一种重要的神经营养蛋白提供有用的诊断工具；此外，它们还展示了核酸参与新型结合相互作用潜力的又一个实例。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c91/307178/836c55ff1b94/nar00016-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c91/307178/836c55ff1b94/nar00016-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c91/307178/836c55ff1b94/nar00016-0131-a.jpg

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