Illangasekare M, Sanchez G, Nickles T, Yarus M
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347.
Science. 1995 Feb 3;267(5198):643-7. doi: 10.1126/science.7530860.
An RNA has been selected that rapidly aminoacylates its 2'(3') terminus when provided with phenylalanyl-adenosine monophosphate. That is, the RNA accelerates the same aminoacyl group transfer catalyzed by protein aminoacyl-transfer RNA synthetases. The best characterized RNA reaction requires both Mg2+ and Ca2+. These results confirm a necessary prediction of the RNA world hypothesis and represent efficient RNA reaction (> or = 10(5) times accelerated) at a carbonyl carbon, exemplifying a little explored type of RNA catalysis.
已筛选出一种RNA，当提供苯丙氨酰-腺苷单磷酸时，它能迅速将其2'（3'）末端氨酰化。也就是说，这种RNA加速了由蛋白质氨酰转移RNA合成酶催化的相同氨酰基转移反应。最具特征的RNA反应需要Mg2+和Ca2+。这些结果证实了RNA世界假说的一个必要预测，并代表了在羰基碳处的高效RNA反应（加速10^5倍或更高），例证了一种较少探索的RNA催化类型。
