烧伤后白细胞黏附分子表达的改变。
Alterations in leukocyte adhesion molecule expression after burn injury.
作者信息
Nwariaku F E, Mileski W J, Lightfoot E, Sikes P J, Lipsky P E
机构信息
Department of Surgery, University of Texas Southwestern Medical Center at Dallas 75235-9031, USA.
出版信息
J Trauma. 1995 Aug;39(2):285-8. doi: 10.1097/00005373-199508000-00016.
OBJECTIVE
To determine if thermal injury alters the expression of leukocyte adhesion molecules.
DESIGN
This is a controlled experimental animal study.
MATERIALS AND METHODS
Partial thickness burns were created on the backs of New Zealand White rabbits. At 30 minutes, 2 hours, 4 hours, and 24 hours after burn, skin was harvested for immunohistochemistry. Monoclonal antibodies were used to study changes in intercellular adhesion molecule 1 (ICAM-1), E-selectin, and leukocyte CD11a. Staining was graded on a scale of 0 to 4.
MEASUREMENTS AND MAIN RESULTS
ICAM-1 was significantly decreased at 24 hours after burn (p < 0.007, Wilcoxon signed rank test). CD11a was increased at 30 minutes (p < 0.02), 2 hours (p < 0.02), and 24 hours (p < 0.006). E-selectin was increased at 2 hours (p < 0.03).
CONCLUSION
Thermal injury alters the expression of leukocyte adhesion molecules.
目的
确定热损伤是否会改变白细胞粘附分子的表达。
设计
这是一项对照实验动物研究。
材料与方法
在新西兰白兔背部造成浅二度烧伤。烧伤后30分钟、2小时、4小时和24小时，取皮肤进行免疫组织化学检查。使用单克隆抗体研究细胞间粘附分子1（ICAM-1）、E-选择素和白细胞CD11a的变化。染色按0至4级评分。
测量结果与主要结果
烧伤后24小时ICAM-1显著降低（p < 0.007，Wilcoxon符号秩检验）。CD11a在30分钟（p < 0.02）、2小时（p < 0.02）和24小时（p < 0.006）时升高。E-选择素在2小时时升高（p < 0.03）。
结论
热损伤会改变白细胞粘附分子的表达。
Zotero 插件