Tonissen K F, Drysdale T A, Lints T J, Harvey R P, Krieg P A
Department of Zoology, University of Texas at Austin 78712.
Dev Biol. 1994 Mar;162(1):325-8. doi: 10.1006/dbio.1994.1089.
We have isolated a Xenopus homeodomain sequence, XNkx-2.5, which shows significant similarity to mouse Nkx-2.5 and to the Drosophila tinman gene product. In Drosophila, tinman is required for formation of the heart and visceral mesoderm structures. In situ hybridization studies show that XNkx-2.5 is expressed in the heart region during early Xenopus development and later is also expressed in gut tissue. The observed similarity of sequences and expression patterns suggests that the regulatory mechanisms underlying heart formation may be conserved between distant species.
我们分离出了一种非洲爪蟾同源异型结构域序列XNkx - 2.5，它与小鼠的Nkx - 2.5以及果蝇的tinman基因产物具有显著的相似性。在果蝇中，tinman对于心脏和内脏中胚层结构的形成是必需的。原位杂交研究表明，XNkx - 2.5在非洲爪蟾早期发育过程中在心脏区域表达，后期也在肠道组织中表达。观察到的序列和表达模式的相似性表明，心脏形成的潜在调控机制可能在远缘物种间保守。
