Franck J P, Kornfield I, Wright J M
Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
Mol Phylogenet Evol. 1994 Mar;3(1):10-6. doi: 10.1006/mpev.1994.1002.
The SATA satellite DNA family of sequences, composed of three size variants of approximately 237, 230, and 209 bp, is conserved in the genomes of tilapiine and haplochromine cichlid fishes. In the present study we examined the utility of the SATA sequences for inferring phylogenetic relationships among the three major genera of tilapiine fishes, Oreochromis, Sarotherodon, and Tilapia. Hybridization of the monomer SATA repeat to genomic DNA of representative cichlid species established conservation of the sequence in the African tilapine and haplochromine lineages and its absence from other cichlid lineages. Bootstrapped DNA parsimony and neighbor-joining analyses of derived consensus sequences revealed two distinct clades, one containing the mouthbrooding genera Oreochromis and Sarotherodon, and the other containing the substrate spawning genus Tilapia. These results are consistent with recent independent studies using mitochondrial DNA and establish the utility of the SATA satellite DNA family for phylogenetic reconstruction. Concerted evolution of the SATA sequences was also demonstrated within the tilapiine tribe.
SATA卫星DNA序列家族由大约237、230和209 bp的三种大小变体组成，在罗非鱼和帚齿非鲫丽鱼科鱼类的基因组中是保守的。在本研究中，我们检验了SATA序列在推断罗非鱼科鱼类三个主要属，即奥利亚罗非鱼属、帚齿罗非鱼属和罗非鱼属之间系统发育关系方面的效用。单体SATA重复序列与代表性丽鱼科物种的基因组DNA杂交，证实了该序列在非洲罗非鱼和帚齿非鲫谱系中的保守性，而在其他丽鱼科谱系中则不存在。对推导的共有序列进行自展DNA简约分析和邻接法分析，揭示了两个不同的分支，一个包含口孵属奥利亚罗非鱼属和帚齿罗非鱼属，另一个包含底物产卵属罗非鱼属。这些结果与最近使用线粒体DNA的独立研究一致，并确立了SATA卫星DNA家族在系统发育重建中的效用。SATA序列的协同进化也在罗非鱼族中得到了证实。
