Takahashi T, Kawamura Y, Sakata N, Elmesiry G E, Takemon Y, Tanida K, Minoshima S, Shimizu N, Kato M
Department of Life Sciences, College of Integrated Arts and Sciences, Osaka Prefecture University, Sakai, Japan.
Mol Biol Rep. 2001;28(2):119-22. doi: 10.1023/a:1017980227068.
We have isolated and sequenced a member of tandem repetitive DNA containing BamHI site (BamHI family satellite DNA) from bluegill sunfish Lepomis macrochirus. PCR amplification with specific primers was performed to define the size of unit length repeat of the BamHI family satellite DNA, revealing that there were two distinct size of DNA fragments (0.9 kb and 1.3 kb) in the PCR products. The longer fragment (1.3 kb) consisted of internal sub-duplication of shorter fragment (0.9 kb). We have compared the size of PCR products among four fish populations, and found that both fragments co-existed in one population whereas the longer fragment was dominant in other three populations. The results may reflect ongoing homogenization of satellite DNA type over a short evolutionary time scale.
我们从蓝鳃太阳鱼（Lepomis macrochirus）中分离并测序了一个含有BamHI位点的串联重复DNA成员（BamHI家族卫星DNA）。使用特异性引物进行PCR扩增，以确定BamHI家族卫星DNA单位长度重复序列的大小，结果显示PCR产物中有两种不同大小的DNA片段（0.9 kb和1.3 kb）。较长的片段（1.3 kb）由较短片段（0.9 kb）的内部重复组成。我们比较了四个鱼类群体中PCR产物的大小，发现两个片段在一个群体中共存，而较长的片段在其他三个群体中占主导地位。这些结果可能反映了在较短的进化时间尺度上卫星DNA类型正在进行的同质化过程。
