一种针对早期妊娠因子诱导的抑制因子（EPF-S1）的单克隆抗体可破坏小鼠的着床过程。
A monoclonal antibody to an early pregnancy factor-induced suppressor factor (EPF-S1) disrupts implantation in mice.
作者信息
Athanasas-Platsis S, Hoskin M J, Rolfe B E, Cavanagh A C, Morton H
机构信息
University of Queensland, Department of Surgery, Royal Birsbane Hospital, Australia.
出版信息
Am J Reprod Immunol. 1995 Mar;33(3):259-66. doi: 10.1111/j.1600-0897.1995.tb00893.x.
PROBLEM
The importance of EPF during pregnancy has been established previously but the importance of the EPF-induced suppressor factor EPF-S1 in pregnancy has to date been unaddressed. Investigations were therefore conducted in order to study this.
METHOD
Monoclonal antibodies to EPF-S1 were produced, and one antibody, designated R2T gamma, was characterized. Mated mice were passively immunized with R2T gamma and the effect on implantation determined.
RESULTS
Characterization of anti-EPF-S1 R2T gamma revealed that it cross-reacted with EPF-S1 of different MHC restriction but not with EPF or EPF-S2. When injected into mated mice on days 1 to 4, R2T gamma had no effect on pregnancy but when injections continued to day 5, pregnancy was affected; the number of embryos implanted on day 7 were significantly less than the number of corpora lutea counted, signifying embryonic loss.
CONCLUSION
These studies show that anti-EPF-S1 R2T gamma disrupts implantation in mice when injected on days 1 to 5 of pregnancy but not when injected on days 1 to 4, demonstrating that EPF-S1 exerts its effects around the time of implantation.
问题
此前已证实早孕因子（EPF）在孕期的重要性，但迄今为止，EPF诱导的抑制因子EPF-S1在孕期的重要性尚未得到探讨。因此开展了相关研究以对此进行探究。
方法
制备了针对EPF-S1的单克隆抗体，并对其中一种名为R2Tγ的抗体进行了特性鉴定。用R2Tγ对交配后的小鼠进行被动免疫，并测定其对胚胎着床的影响。
结果
抗EPF-S1的R2Tγ特性鉴定显示，它能与不同主要组织相容性复合体（MHC）限制的EPF-S1发生交叉反应，但不与EPF或EPF-S2发生反应。在第1至4天注射到交配后的小鼠体内时，R2Tγ对妊娠没有影响，但当注射持续到第5天时，妊娠受到影响；第7天着床的胚胎数量明显少于计数的黄体数量，表明胚胎丢失。
结论
这些研究表明，抗EPF-S1的R2Tγ在妊娠第1至5天注射时会破坏小鼠的胚胎着床，但在第1至4天注射时则不会，这表明EPF-S1在胚胎着床前后发挥作用。
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