以马鹿（ Cervus elaphus ）作为延迟着床模型合成早期妊娠因子 。
Synthesis of early pregnancy factor using red deer (Cervus elaphus) as a delayed implantation model.
作者信息
Lash G E, Legge M, Fisher M
机构信息
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
出版信息
J Assist Reprod Genet. 1997 Jan;14(1):39-43. doi: 10.1007/BF02765751.
PURPOSE
This study measured serum early pregnancy factor (EPF) in pregnant red deer (Cervus elaphus) and ascertained whether EPF synthesis is associated with implantation.
METHODS
Serial serum samples were taken from mated hinds up to 42 days postconception and analyzed for EPF activity using the rosette inhibition test. EPF activity was then correlated with calving records and stages of preimplantation development.
RESULTS
EPF was detected in all pregnant animals, with a twin pregnancy giving increased EPF activity. Three animals gave an EPF response following fertilization but failed to continue beyond the preimplantation embryo stage. The increase in EPF synthesis previously associated with implantation in other mammals occurred at the blastocyst stage in red deer.
CONCLUSIONS
EPF synthesis in red deer (Cervus elaphus) is consistent with the preimplantation period, as occurs in other mammals. However, the second phase of the biphasic increase in early pregnancy factor production is associated with blastocyst formation, not implantation.
目的
本研究检测了怀孕马鹿（ Cervus elaphus ）血清中的早期妊娠因子（EPF），并确定EPF的合成是否与着床有关。
方法
在受孕后长达42天的时间内，从交配后的母鹿采集系列血清样本，使用玫瑰花结抑制试验分析EPF活性。然后将EPF活性与产犊记录和着床前发育阶段进行关联分析。
结果
在所有怀孕动物中均检测到EPF，怀有双胎的动物EPF活性增加。三只动物在受精后出现了EPF反应，但在着床前胚胎阶段之后未能继续妊娠。先前在其他哺乳动物中与着床相关的EPF合成增加在马鹿中发生于囊胚期。
结论
马鹿（ Cervus elaphus ）中的EPF合成与其他哺乳动物一样，与着床前期一致。然而，早期妊娠因子产生的双相增加的第二阶段与囊胚形成有关，而非着床。
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