Liu S M, Jones W J
University of Georgia, Athens 30602, USA.
Appl Microbiol Biotechnol. 1995 Aug-Sep;43(4):725-32. doi: 10.1007/BF00164780.
Nonadapted freshwater sediment slurries and sediment slurries adapted to dechlorinate 2,3-dichloropyridine (2,3-Cl2Pyd), 2,3-dichloroaniline (2,3-Cl2Anl), 2,3-dichlorophenol (2,3-Cl2PhOH), 3,5-dichloropyridine (3,5-Cl2Pyd), 3,5-dichloroaniline (3,5-Cl2Anl) and 3,5-dichlorophenol (3,5-Cl2PhOH) were studied to determine the rate, range and extent of biotransformation of structurally related compounds under anaerobic conditions. 2,3-dichloroanisole (2,3-Cl2Ans) and 3,5-dichloroanisole (3,5-Cl2Ans) were initially demethylated, producing 2,3-Cl2PhOH and 3,5-Cl2PhOH as intermediate transformation products. All other dichloroaromatic compounds examined were initially dechlorinated. The rates of dechlorination of 2,3-Cl2PhOH, 2,3-Cl2Anl, and 2,3-Cl2Pyd were significantly lower (5-15 times) in nonadapted sediment slurries compared to sediment slurries adapted to 2,3-Cl2Anl or 2,3-Cl2Pyd. In 2,3-Cl2PhOH adapted sediment, the rate of dechlorination of 2,3-Cl2PhOH was 15 times greater than in nonadapted sediment; however, the rates of dechlorination of 2,3-Cl2Anl and 2,3-Cl2Pyd were similar for 2,3-Cl2PhOH-adapted and nonadapted sediment slurries. In adapted and nonadapted sediment slurries, 2,3-Cl2PhOH, 2,3-Cl2Anl, and 2,3-Cl2Pyd were preferentially dechlorinated at the ortho, meta, and meta positions, respectively. Additionally, 2,3-Cl2Pyd adapted sediment slurries dechlorinated 2,3-Cl2PhOH and 2,3-Cl2Pyd at both ortho and meta positions. Rates of dechlorination of 3,5-Cl2PhOH, 3,5-Cl2Anl, and 3,5-Cl2Pyd were lower (2-4 times) in nonadapted sediment slurries compared to sediment slurries adapted to 3,5-Cl2Anl or 3,5-Cl2Pyd. In 3,5-Cl2PhOH adapted sediment, the rate of dechlorination of 3,5-Cl2PhOH was approximately 10 times greater than in nonadapted sediment.(ABSTRACT TRUNCATED AT 250 WORDS)
研究了未适应的淡水沉积物浆液以及适应于对2,3 - 二氯吡啶（2,3 - Cl₂Pyd）、2,3 - 二氯苯胺（2,3 - Cl₂Anl）、2,3 - 二氯苯酚（2,3 - Cl₂PhOH）、3,5 - 二氯吡啶（3,5 - Cl₂Pyd）、3,5 - 二氯苯胺（3,5 - Cl₂Anl）和3,5 - 二氯苯酚（3,5 - Cl₂PhOH）进行脱氯的沉积物浆液，以确定厌氧条件下结构相关化合物的生物转化速率、范围和程度。2,3 - 二氯苯甲醚（2,3 - Cl₂Ans）和3,5 - 二氯苯甲醚（3,5 - Cl₂Ans）最初发生脱甲基反应，生成2,3 - Cl₂PhOH和3,5 - Cl₂PhOH作为中间转化产物。所检测的所有其他二氯芳香族化合物最初都发生了脱氯反应。与适应2,3 - Cl₂Anl或2,3 - Cl₂Pyd的沉积物浆液相比，未适应的沉积物浆液中2,3 - Cl₂PhOH、2,3 - Cl₂Anl和2,3 - Cl₂Pyd的脱氯速率显著更低（低5 - 15倍）。在适应2,3 - Cl₂PhOH的沉积物中，2,3 - Cl₂PhOH的脱氯速率比未适应的沉积物高15倍；然而，对于适应2,3 - Cl₂PhOH和未适应的沉积物浆液，2,3 - Cl₂Anl和2,3 - Cl₂Pyd的脱氯速率相似。在适应和未适应的沉积物浆液中，2,3 - Cl₂PhOH、2,3 - Cl₂Anl和2,3 - Cl₂Pyd分别优先在邻位、间位和间位发生脱氯反应。此外，适应2,3 - Cl₂Pyd的沉积物浆液在邻位和间位对2,3 - Cl₂PhOH和2,3 - Cl₂Pyd进行脱氯。与适应3,5 - Cl₂Anl或3,5 - Cl₂Pyd的沉积物浆液相比，未适应的沉积物浆液中3,5 - Cl₂PhOH、3,5 - Cl₂Anl和3,5 - Cl₂Pyd的脱氯速率更低（低2 - 4倍）。在适应3,5 - Cl₂PhOH的沉积物中，3,5 - Cl₂PhOH的脱氯速率比未适应的沉积物大约高10倍。（摘要截短于250字）
