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半松叶蜂属(膜翅目:叶蜂科)中一个串联重复DNA序列家族(pYS家族)的分子特征分析

Molecular characterization of a family of tandemly repetitive DNA sequences (pYS family) in the genus Hemitaxonus (Hymenoptera: Tenthredinidae).

作者信息

Sonoda S, Yamada T, Naito T, Nakasuji F

机构信息

Laboratory of Applied Entomology, Faculty of Agriculture, Okayama University, Tsushima, Japan.

出版信息

Jpn J Genet. 1995 Aug;70(4):533-42. doi: 10.1266/jjg.70.533.

DOI:10.1266/jjg.70.533
PMID:7546846
Abstract

Distribution of a family of tandemly repetitive sequences in Hemitaxonus japonicus (pYS family) was investigated among eight sawfly species and host races in the genus Hemitaxonus. High copy numbers of the repetitive sequences were detected in the two host plant races of H. japonicus, race Polystichum polyblepharum (race PP) and race P. tripteron (race PT), and H. sasayamensis, whereas a low copy number in H. minomensis. Comparison of the nucleotide sequences of the basic repeat units demonstrated a high degree of homology among the four tested species with high AT-rich sequences. Minor repeat units shorter than the consensus basic repeat unit of pYS family were found only in races PP and PT of H. japonicus. Analyses of their nucleotide sequences showed the occurrence of the host race specific sub-repeat units.

摘要

在半松叶蜂属的8种锯蜂和寄主类群中,研究了日本半松叶蜂(pYS家族)中一个串联重复序列家族的分布情况。在日本半松叶蜂的两个寄主植物类群,即多羽耳蕨类群(PP类群)和三叉耳蕨类群(PT类群)以及佐山半松叶蜂中,检测到了高拷贝数的重复序列,而米诺半松叶蜂中的拷贝数较低。对基本重复单元的核苷酸序列进行比较表明,在这四个具有高AT含量序列的测试物种之间存在高度同源性。仅在日本半松叶蜂的PP类群和PT类群中发现了比pYS家族共有基本重复单元短的次要重复单元。对其核苷酸序列的分析表明存在寄主类群特异性的亚重复单元。

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