Barry J L, Hoebler C, Macfarlane G T, Macfarlane S, Mathers J C, Reed K A, Mortensen P B, Nordgaard I, Rowland I R, Rumney C J
INRA, Laboratory of Applied Technology and Nutrition, Nantes, France.
Br J Nutr. 1995 Sep;74(3):303-22. doi: 10.1079/bjn19950137.
Five European laboratories tested a simple in vitro batch system for dietary fibre fermentation studies. The inoculum was composed of fresh human faeces mixed with a carbonate-phosphate buffer complex supplemented with trace elements and urea. Five dietary fibre sources (cellulose, sugarbeet fibre, soyabean fibre, maize bran and pectin) were used by each laboratory on three occasions to determine pH, residual non-starch polysaccharides (NSP) and short-chain fatty acid production during fermentation. Cellulose and maize bran degradabilities were very low (7.2(SE 10.8) and 6.2 (SE 9.1)% respectively after 24 h), whereas pectin and soyabean fibre were highly degraded (97.4 (SE 4.4) and 91.1 (SE 3.4)% respectively after 24 h). Sugarbeet fibre exhibited an intermediate level of degradability (59.5 (SE 14.9)%). Short-chain fatty acid production was closely related to NSP degradation (r 0.99). Although each variable was ranked similarly by all laboratories, some differences occurred with respect to absolute values. However, the adaptation of donors to the experimental substrates was not an influential factor. Interlaboratory differences could be reduced either by adding less substrate during incubations or using less-diluted inocula. In vitro fermentations with inocula made from human faeces and from rat caecal contents gave similar results. There was a close correspondence between the data obtained in the present experiment and those previously published in in vivo studies in the rat using the same fibres. The in vitro batch system tested during the present study provides a rapid means of obtaining quantitative estimates of the fermentation and the estimation of the energy content of new sources of dietary fibre.
五个欧洲实验室对一种用于膳食纤维发酵研究的简单体外批次系统进行了测试。接种物由新鲜人类粪便与补充了微量元素和尿素的碳酸盐 - 磷酸盐缓冲复合物混合而成。每个实验室在三个不同时间使用了五种膳食纤维来源（纤维素、甜菜纤维、大豆纤维、玉米麸皮和果胶），以测定发酵过程中的pH值、残余非淀粉多糖（NSP）和短链脂肪酸的产生。纤维素和玉米麸皮的降解率非常低（24小时后分别为7.2（标准误10.8）%和6.2（标准误9.1）%），而果胶和大豆纤维的降解程度很高（24小时后分别为97.4（标准误4.4）%和91.1（标准误3.4）%）。甜菜纤维表现出中等程度的降解性（59.5（标准误14.9）%）。短链脂肪酸的产生与NSP的降解密切相关（r = 0.99）。尽管所有实验室对每个变量的排序相似，但在绝对值方面存在一些差异。然而，供体对实验底物的适应性不是一个有影响的因素。通过在培养过程中减少底物添加量或使用稀释度较低的接种物，可以减少实验室间的差异。用人粪便和大鼠盲肠内容物制成的接种物进行的体外发酵得到了相似的结果。本实验获得的数据与先前在大鼠体内使用相同纤维进行的研究中发表的数据密切相关。本研究中测试的体外批次系统提供了一种快速获得膳食纤维发酵定量估计值以及估计新膳食纤维来源能量含量的方法。
