Czajkowsky D M, Huang C, Shao Z
Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, USA.
Biochemistry. 1995 Oct 3;34(39):12501-5. doi: 10.1021/bi00039a003.
In a solution of phosphate-buffered saline (PBS), unilamellar bilayers with saturated phosphatidylcholines in one leaflet and negatively charged, unsaturated phospholipids in the other leaflet were observed in the ripple phase at room temperature using atomic force microscopy (AFM). This is the first observation of the ripple phase in asymmetric bilayers. Sodium and phosphate, components of PBS, were found to be necessary for the formation of the ripple structure in the asymmetric bilayers composed of dipalmitoylphosphatidylcholine (DPPC) and 1-palmitoyl-2-oleoylphosphatidylglycerol (POPG), demonstrating a dependency for specific ions for this phase. These results indicate that the two leaflets of a bilayer are closely coupled to give rise to such a long range and complicated morphology.
在磷酸盐缓冲盐水（PBS）溶液中，室温下使用原子力显微镜（AFM）在波纹相中观察到单分子层双分子膜，其中一个小叶含有饱和磷脂酰胆碱，另一个小叶含有带负电荷的不饱和磷脂。这是首次在不对称双分子膜中观察到波纹相。发现PBS的成分钠和磷酸盐对于由二棕榈酰磷脂酰胆碱（DPPC）和1-棕榈酰-2-油酰磷脂酰甘油（POPG）组成的不对称双分子膜中波纹结构的形成是必需的，这表明该相依赖于特定离子。这些结果表明，双分子膜的两个小叶紧密耦合，从而产生这种长程且复杂的形态。
