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三(羟甲基)氨基甲烷(C4H11NO3)在支持的单层磷脂双分子层中诱导出波纹相。

Tris(hydroxymethyl)aminomethane (C4H11NO3) induced a ripple phase in supported unilamellar phospholipid bilayers.

作者信息

Mou J, Yang J, Shao Z

机构信息

Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville 22908.

出版信息

Biochemistry. 1994 Apr 19;33(15):4439-43. doi: 10.1021/bi00181a001.

DOI:10.1021/bi00181a001
PMID:8161497
Abstract

A commonly used buffer compound, tris(hydroxymethyl)aminomethane (C4H11NO3), was found to induce a ripple phase in supported unilamellar phospholipid bilayers at room temperature. The ripple structure showed various types of domains that could extend to several micrometers in length with many well-defined bendings of either 120 degrees or 60 degrees. Two different periodic ripples were found to coexist in 1,2-dipentadecanoyl-sn-glycero-3-phosphatidylcholine bilayers. Some intermediate states during the transition to the ripple phase were also observed.

摘要

一种常用的缓冲化合物,三(羟甲基)氨基甲烷(C4H11NO3),被发现在室温下能在支撑的单层磷脂双分子层中诱导出波纹相。波纹结构显示出各种类型的畴,其长度可延伸至几微米,有许多明确的120度或60度弯曲。在1,2-二五烷酰基-sn-甘油-3-磷脂酰胆碱双分子层中发现两种不同的周期性波纹共存。还观察到了向波纹相转变过程中的一些中间状态。

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