Rachinskii V V, Davidova E G, Kretova L G
Biol Bull Acad Sci USSR. 1978 Sep-Oct;5(5):634-8.
An investigation of the isotopic exchange (turnover) of carbon among the basic groups of organic substances of yeast biomass (proteins, polysaccharides, lipids, free amino acids, carbohydrates, and organic aicds) showed that in the presence of a carbon source and all the necessary nutrient elements in the logarithmic phase of growth of the culture, the bulk of the organic substances of the cell are extremely stable. Under these conditions, no appreciable utilization of the carbon of low-molecular-weight pools, nor any significant degradation of high-molecular-weight substances can be detected. Nor was there any redistribution of carbon-14 among individual components of the fraction of free amino acids and lipids. It was shown that the fraction of exchangeable carbon of the biopolymers of mature cells, like the fraction of the exchnage pool of low-molecular-weight substances, does not exceed 10% of the total carb-n of these substances. It is suggested that there is no mixing of the old and newly synthesized low-molecular-weight carbon pools in the parent cell, and all the components of the biomass of the daughter cells are synthesized chiefly from the carbon of the substrate.
对酵母生物质（蛋白质、多糖、脂质、游离氨基酸、碳水化合物和有机酸）有机物质基本基团间碳的同位素交换（周转）进行的一项研究表明，在培养物生长的对数期存在碳源和所有必需营养元素的情况下，细胞的大部分有机物质极其稳定。在这些条件下，未检测到低分子量库中的碳有明显利用，也未检测到高分子量物质有任何显著降解。在游离氨基酸和脂质部分的各个组分之间也没有碳 - 14 的重新分布。结果表明，成熟细胞生物聚合物的可交换碳部分，如同低分子量物质交换库的部分一样，不超过这些物质总碳量的10%。有人提出，亲代细胞中旧的和新合成的低分子量碳库没有混合，子细胞生物质的所有组分主要由底物中的碳合成。
