Mirzadeh H, Katbab A A, Khorasani M T, Burford R P, Gorgin E, Golestani A
Polymer Research Center of Iran, Tehran.
Biomaterials. 1995 May;16(8):641-8. doi: 10.1016/0142-9612(95)93862-8.
With the purpose of improved tissue compatibility, ethylene-propylene rubber (EPR)-based vulcanizates have been surface grafted with acrylamide (AAm) and 2-hydroxyethyl methacrylate (HEMA) using CO2-pulsed laser as excitation source. Grafted surfaces were characterized by performing scanning electron microscopy combined with energy dispersive X-ray analysis and attenuated total reflectance infrared spectroscopy to study the surface morphology and grafting. Surface hydrophilicity (measured by water drop contact angle) increased for the grafted samples. Fractal type of morphology is formed by the grafted poly(AAm) and poly(HEMA) chains on the surface of EPR, which provides both hydrophilic and hydrophobic sites. In vivo tissue compatibility was assessed by implanting test samples in the deep intramuscular and peritoneal layers of rabbits. After 8 weeks of implantation, comparative results indicate that the adhesion of macrophages to EPR samples modified with AAm and HEMA, with no respiratory burst and cellular damage, is significantly lower than their adhesion on unmodified surfaces which show an activated state of the attached macrophages. Also, no acute or chronic inflammatory reaction was observed at the site of implantation and a thinner fibrous tissue capsule formed around the modified samples, whereas foreign body giant cells adhered to unmodified EPR.
为了提高组织相容性，以乙烯-丙烯橡胶（EPR）为基础的硫化橡胶已使用二氧化碳脉冲激光作为激发源，在其表面接枝了丙烯酰胺（AAm）和甲基丙烯酸2-羟乙酯（HEMA）。通过结合能量色散X射线分析的扫描电子显微镜和衰减全反射红外光谱对接枝表面进行表征，以研究表面形态和接枝情况。接枝样品的表面亲水性（通过水滴接触角测量）有所增加。接枝的聚（AAm）和聚（HEMA）链在EPR表面形成分形形态，这提供了亲水和疏水位点。通过将测试样品植入兔子的深部肌肉层和腹膜层来评估体内组织相容性。植入8周后，比较结果表明，巨噬细胞对用AAm和HEMA改性的EPR样品的粘附，在没有呼吸爆发和细胞损伤的情况下，明显低于其在未改性表面上的粘附，未改性表面上附着的巨噬细胞呈现活化状态。此外，在植入部位未观察到急性或慢性炎症反应，并且在改性样品周围形成了较薄的纤维组织囊，而异物巨细胞粘附在未改性的EPR上。
